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Figure 1 setd5 is expressed in the zebrafish adult brain and at early stages of zebrafish embryo development. (a) setd5-203 isoform localization on zebrafish WT brain sections at 10/12 months of age performing in situ hybridization technique. Representative images of a section corresponding to telencephalon (1–3), diencephalon-mesencephalon (4,5) and rhombencephalon (6), n = 3 brains analyzed. Scale bar 300 µm. (b) Expression of setd5 isoforms in zebrafish embryos and larvae at different developmental stages, obtained by RT-qPCR analysis. Data are expressed as 2−(ΔCt) mean ± Standard Error of the Mean (SEM), using b-actin1 as housekeeping gene. n = 3 independent experiments. Abbreviations: D, Dorsal telencephalic area; V, Ventral telencephalic area; OB, Olfactory bulb; LOT, Lateral olfactory tract; Ppa, Parvocellular preoptic nucleus; TL, Longitudinal Torus; Val, Lateral division of valvular cerebelli; TeO, Tectum opticum; PGZ, Periventricular gray zone of optic tectum; DTN, Dorsal tegmental nucleus; HV, Ventral zone of periventricular hypothalamus; PTN, Posterior tuberal nucleus; PGm, Medial progromerular nucleus; DIL, Diffuse nucleus of the inferior lobe; Hc, Caudal zone of periventricular hypothalamus; Hd, Dorsal zone of the periventricular hypothalamus; CCe, Cerebellar corpus; LCa, Caudal lobe of cerebellum; LVII, Facial lobe; ALLN, Anterior lateral line nerves; VIII, Octaval nerve; hpf, hours post fertilization