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Figure 4 Hypertrophic hearts are evident in ALKO zebrafish in the larvae/adult stage. (A) Defective Atgl enlarged the ventricle size in ALKO at 5 dpf. Scale bar: 500 μm. The relative ventricle area was measured by the ventricle area of the control and ALKO 5 dpf embryo. Percentage of ventricle volume area in Ctrl = 100 ± 8.0138, and ALKO = 118.6974 ± 12.1794. (Control, n = 9; ALKO, n = 11). (B) Upper: The later view of the hearts in control and ALKO at 5 mpf. Lower: Paraffin-embedded sections of the hearts of control and ALKO at 5 mpf with Masson trichrome stained. Scale bar: 200 μm. B.A.: bulbus arteriosus, V: ventricle. (C) Molecule analysis of ALKO demonstrated the downregulation of sarcomere genes in the ventricle of 5 mpf ALKO (n = 3). Values of myh7l = 0.7088 ± 0.0564, actc1b = 0.3882 ± 0.145, mybpc3 = 0.6449 ± 0.0474, and tnnt2a = 0.7824 ± 0.0671 in ALKO group. Statistically significant differences from the controls are indicated by ** p < 0.01, and *** p < 0.001.