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Fig. 3 a–d Zebrafish larval forebrain neuroanatomy shown in transverse Hu-protein stained sections. a Commissural telencephalon with early migrated telencephalic area M4. b Postcommissural telencephalon with early migrated area of eminentia thalami M3. c Rostral diencephalon with early migrated posterior tubercular area M2 (preglomerular complex) and early migrated pretectal area M1. d Caudal diencephalon with early migrated posterior tubercular area M2 (preglomerular complex). e Summary of larval diencephalic zebrafish gene expression patterns (see text for details). f Posteroventral hypothalamic level shows lateral and posterior ventricular recess. Panels (a–f) modified from Mueller and Wullimann [2016]; see there for full account. a1–b1 Corresponding sections with diagnostic regulatory gene markers to identify larval zebrafish migrated areas M4 and M3 [panels modified from Mueller et al., 2008]. ac, anterior commissure; ALLG, anterior lateral line ganglion; bHLH, basic helix-loop-helix; DT, dorsal thalamus; E, epiphysis; EmT, eminentia thalami; Ha, habenula; Hc, Hi, Hr, caudal, intermediate, rostral periventricular hypothalamic zone; lfb, lateral forebrain bundle; LR, lateral ventricular recess of periventricular hypothalamus; M1, early migrated pretectal aera; M2, early migrated posterior tubercular area (preglomerular complex); M3, early migrated area of eminentia thalami; M4, early migrated telencephalic area; mlf, medial longitudinal fascicle; MO, medulla oblongata; oc, optic chiasma; P, pallium; pc, posterior commissure; Po, preoptic area; poc, postoptic commissure; Pr, pretectum; PR, posterior ventricular recess of periventricular hypothalamus; PT, posterior tuberculum; Sdp, posterior subdivision of dorsal part of subpallium (subpallial amygdala homolog); SPV, supraopto-paraventricular region; T, midbrain tegmentum; TeO, tectum opticum; TG, trigeminal ganglion; TS, torus semicircularis; ZLI, zona limitans intrathalamica. For gene names see text.