Figure 7

(A) ISH for exocrine pancreas marker cpa5 and hepatocyte marker fabp10a. EtOH (1%) (12–72 hpf) reduced exocrine pancreas and liver size. (B and C) ImageJ quantification of pancreas and liver size (****P ≤ 0.0001, unpaired 2-tailed t test, n ≥13). (D and E) ISH for exocrine pancreas marker trypsin and hepatocyte markers prox1a and fabp10a in psmb1^hi2939 and psmc6^hi3593 (72 hpf) mutants. (F–I) Quantification of pancreas and liver size using ImageJ. psmb1^+/–, psmb1^–/–, and psmc6^–/– have significantly reduced exocrine pancreas size. psmb1^–/– and psmc6^–/– have significantly reduced liver size (n > 4, ****P ≤ 0.0001, **P < 0.01, ordinary 1-way ANOVA with Dunnett’s multiple-comparison test). (J–L) ISH for liver marker fabp10a and exocrine pancreas marker trypsin, followed by quantification of organ size using ImageJ area calculation at 96 hpf. EtOH (1%) (12–96 hpf) significantly reduced liver and pancreas size. Treatment with BTZ significantly exacerbated the effects of EAE on liver and pancreas area (*P < 0.05, **P < 0.01, ***P < 0.001, 2-way ANOVA with Sidak’s multiple comparisons). BTZ concentration = 2.5 μM (12–96 hpf). Scale bars: 100 μm. From left, column sample n = 7, 16, 13 (F); n = 6, 20, 7 (G); n = 6, 20, 7 (H); n = 5, 12, 12 (I); n = 19, 21, 25, 19 (K); and n = 19, 19, 15, 25 (L). Data represent mean ± SD.