Figure 4

Effect of CARD14 overexpression on gene expression. Embryos were injected with expression vectors carrying human wild type and PRP-causing CARD14 mutation (p.Glu138del). Relative expression of nfkb2, ccl20b, vangl1 and vangl2 was ascertained 1 dpf using qPCR (upper panel). Results represent the mean of 3 replicates and are provided as gene expression relative to gene expression in control embryos ± standard error normalized to ppiab mRNA levels (two-sided t-test; ** p < 0.01, *** p < 0.001). Protein interaction (STRING, http://string-db.org, accessed on 17 May 2019) analysis of CARD14 and planar cell polarity proteins in zebrafish (left lower panel) and human (right lower panel) is predictive of a functional interaction between CARD14 and VANGL2.