Figure 6
In vitro uptake of anti‐miR223 protocells by human macrophages enhances expression of pro‐inflammatory markers. A) Schematic of the experimental design; monocytes isolated from human donors undergo in vitro differentiation toward macrophages (via M‐CSF) prior to FITC‐protocell addition (day 7). B) Multi‐channel confocal images of human macrophages after incubation with different FITC‐protocell concentrations for 3 h; white arrowheads indicate protocells within macrophages. C) Imaris 3D reconstruction from confocal movie frames showing FITC‐protocell uptake by a human macrophage 2 h after protocell addition (blue dashed box in [B]). See also Movie