Distribution of immune cells during EIU inflammation process in zebrafish. (A) L-plastin (magenta), Mpx (green), and DAPI (blue) staining was seen in cryosections at 0 hpi (blank control), 4 hpi, 12 hpi, 24 hpi and 72 hpi of LPS. ONL, outer nuclear layer, INL, inner nuclear layer, IPL, inner plexiform layer, GCL, ganglion cell layer. Asterisk: L-plastin+/Mpx- cell. White arrow: L-plastin+/Mpx+ cell. Scale bar: 20μm. (B) Quantification of immune cells in the iris and vitreous body at each time point (mean ± SD; *P < 0.05, **P < 0.01, ****P < 0.0001, the number of L-plastin+/Mpx- cells were compared; †P < 0.05, ††P < 0.01, number of L-plastin+/Mpx+ cells were compared, one-way ANOVA).
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