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Fig. 2
Microglia are significantly less efficient in the uptake of dextran and Transferrin from the brain parenchyma compared to BLECs. (a) Schematic overview of the dorsal head region of a zebrafish embryo indicating the zoom-in area in (b-d, e, h). (b-d) Maximum projection showing the characteristic loops of lyve1:DsRed positive BLECs on top of the optical tecta and indicating the position of mpeg:GFP positive microglia at 5dpf. (e-j) mpeg:GFP positive zebrafish embryo in which pHr-dextran (e) and Transferrin-647 (h) were co-injected into the brain parenchyma. Arrows highlight an uptake of the respective substance by BLECs. (f, g) and (i, j) zoom-in of the boxed region in (e) and (h), respectively. Arrowheads indicate cargo uptake in a macrophage. (k) Quantification of the average pixel intensity of pHr-dextran shows a significantly higher uptake in BLECs compared to microglia (Welch's t-test, p = .0044). (l) The average pixel intensity for Transferrin-647 in BLECs is significantly higher compared to microglia (Welch's t-test, p = .0004). Number of analyzed embryos was n = 4. Values are presented as means ± SD
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