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Fig. 4
BLECs ingest HPV-16 pseudoviruses more efficiently than microglia. (a-d) Maximum projection of a mpeg:GFP positive zebrafish embryo that was co-injected with pHr-HPV-16 and IgG-Alexa647 into the CSF at 5dpf. The white box indicates the zoom-in area in (e-h). Single channel of maximum projection in (a) for mpeg:GFP (b), pHr-HPV-16 (c) and IgG-Alexa647 (d). Arrowheads highlight uptake into BLECs while arrows mark endocytosis of the respective substrate in microglia. Note the auto-fluorescence of pigment cells in the red channel, marked by asterisks. (i, j) Quantification of the average pixel intensities within mpeg:GFP+ microglia and BLECs in n = 12 embryos shows a significantly higher uptake of pHr-HPV-16 (Welch's t-test, p = .0097) and IgG-Alexa647 (Welch's t-test, p = .0001) in BLECs when compared to microglia. Values are presented as means ± SD
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