ZFIN Image: Geng et al., 2022, Fig. 5.

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Figure Caption

Fig. 5.

RNA-seq reveals down-regulation of autism risk genes caused by Top2a depletion.

(A) RNA-seq experimental design. (B) can4Dn, but not can4Up, is selectively enriched for autism and its comorbidities risk genes. For each cell, color represents odds ratio. Numbers in the top six rows represent ranking out of 24,166 diseases in the DisGeNET library (before slash) and adjusted P value (after slash). Numbers in the bottom three rows represent adjusted P value. SFARI_1/2, high-confidence SFARI genes with a gene score of 1 or 2. (C and D) GSEA analysis using the DisGeNET library shows enrichment for autism and its comorbidities risk genes (C) and neurological condition risk genes (D) in down-regulated. Value inside each bar represents false discovery rate (FDR) (C). Significance: FDR < 5%. (E) qPCR shows the down-regulation of Satterstrom genes in the brain of adult zebrafish that received embryonic treatment of 15 μM flumequine or 100 μM sodium salicylate compared to DMSO control. ****P < 0.0001.

Acknowledgments

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