ZFIN Image: Geng et al., 2022, Fig. 3.

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Figure Caption

Fig. 3.

Top2a, rather than Top2b, is required for the development of social behavior.

(A) Top2a-MO (0.05 mM; n = 44) induces social deficits compared to vehicle (H₂O, n = 44), which is rescued by hTOP2A mRNA (250 ng/μl; Top2a-MO + A, n = 44). (B) Top2b-MO (0.05 mM; n = 50) does not induce social deficits compared to vehicle (H₂O, n = 71). (C) Top2a-selective inhibitor sodium salicylate (100 μM) induces social deficits. (D) Heterozygous can4 mutant (can4^+/−, n = 20) exhibits social deficits compared to WT (n = 20). Both mutant and WT were acquired from a heterozygous (can4^+/−) incross and genotyped individually after Fishbook assay. (E) Heterozygous noto mutant (noto^+/−, n = 32) shows no social deficits compared to WT (n = 21). Both mutant and WT were acquired from a heterozygous (noto^+/−) incross and genotyped individually after Fishbook assay. (F) ICRF-193 (I; 0.1 mM; n = 23) induced social deficits compared to the vehicle control ethanol (E; 1%; n = 50), which is rescued by hTOP2AY165S mRNA (250 ng/μl; I + A^Y165S, n = 31). ns, not significant; *P < 0.05, **P < 0.01, and ****P < 0.0001.

Acknowledgments

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