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Figure 6 Effect of MitoTEMPO on doxorubicin-induced osteoclast differentiation and oxidative stress markers. RAW264.7 cells were cultured for 4 days with M-CSF (30 ng/mL) and RANKL (50 ng/mL) and treated with MitoTEMPO (MT) and Doxorubicin (DOX) alone or together. Levels of expression for Oc-stamp (A), Rank (B), Trap (C), Ctsk (D), Nfatc1 (E), Nf-kb p105 (F), FoxM1 (G), Sod1 (H) and Nrf 2 (I) were analyzed by qPCR. One-way ANOVA, Tukey’s multiple comparisons test, *—p ≤ 0.05, **—p ≤ 0.01, ***—p ≤ 0.001, ****—p ≤ 0.0001.
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