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Figure 5 Effect of MitoTEMPO on doxorubicin-induced osteoclast differentiation. RAW 264.7 cells were cultured for 4 days with M-CSF (30 ng/mL) and RANKL (50 ng/mL) and treated with MitoTEMPO (MT) and Doxorubicin (DOX) alone or together. The cells were stained for TRAP (A). The number of TRAP-positive cells (B). Cells were grouped according to number of nuclei: 1, 2, 3 or more than 4 (≥4) (C). One-way ANOVA, Tukey’s multiple comparisons test, *—p ≤ 0.05, **—p ≤ 0.01, ***—p ≤ 0.001, ****—p ≤ 0.0001.
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Full text @ Int. J. Mol. Sci.