Figure 2.

Figure 2.

Algorithm for determining the signal strengths of the different cone types contributing to ERG spectral data. A, Equation 1. Aspartate-isolated cone signals from zebrafish eyes are a summation of signals from eight cone types, each distinguished by different maximal amplitudes (Vm_i), semi-saturation irradiances (k_i), and opsin peak absorbances (wlmax_i). There are 255 unique combinations of eight cones, each combination is a candidate to best model cone spectral responses in the ERG. B, Spectral shapes of opsin absorbances [A(wlmax_i, wl)] are generated from 8th-order template polynomials (Palacios et al., 1996; Hughes et al., 1998) using Dartnall nomogram translations along the wavelength axis to represent opsins of different peak wavelengths (Dartnall, 1953). C, Parameters for each of the i cone types are numbered in order from short to long wavelengths. SWS, short-wavelength-sensitive opsins; RH2, rhodopsin-like green-cone opsins; LWS, long-wavelength-sensitive opsins.