ZFIN Image: Seo et al., 2022, Figure 3

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Figure Caption

Figure 3 The loss of tamalin function causes cell death of oligodendrocyte lineage cells in the postembryonic zebrafish spinal cord to adulthood. The representative images are transverse sections of the spinal cord of post-embryonic zebrafish. The dorsal side is displayed at the top. (a–f) Immunolabeling of wildtype (a–c) and tamalin KO (d–f) zebrafish with anti-Olig2 antibody (green) and TUNEL staining (red). (j–o) Immunolabeling of wildtype (j–l) and tamalin KO zebrafish (m–o) with anti-PCNA (green) and anti-Olig2 (red) antibodies. Quantification of the number of TUNEL⁺ Olig2⁺ cells (g–i) and PCNA⁺ Olig2⁺ cells (p–r) at 1 mpf (g,p), 2 mpf (h,q), and 4 mpf (i,r). (** p = 0.002 **** p < 0.0001, n = 10 sections from five zebrafish). Scale bars: (a–l), 20 μm. KO, knockout; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; PCNA, proliferating cell nuclear antigen; ns; no significance and mpf; months post fertilization.

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Acknowledgments

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