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Figure 1 Tamalin is expressed in the neurons but not in the oligodendrocytes. (a–c’) The representative images are transverse sections of the spinal cord. The dorsal side is displayed at the top. (a) Whole-mount in situ RNA hybridization with tamalin mRNA in the spinal cord of wildtype zebrafish at 3 dpf. (b,b’) Immunolabeling of wildtype zebrafish with anti-Hu and anti-tamalin antibodies, and (c,c’) the immunolabeling of Tg(mbp:egfp) zebrafish with an anti-Tamalin antibody at 5 dpf. (b’,c’) High magnification images of the boxes in (b,c). Asterisks indicate the tamalin+ cells. Scale bars: (a–c’), 10 μm. EGFP, Enhanced green fluorescent protein; dpf, days post fertilization.