Figure 6
Upregulated wnk1a, stk39, and osr1b, and downregulated ppp2r1ba and ppp2r1bb in 15 dpf [tert] and [tert x p53−/−] transgenic fish. The expression of wnk1a and its downstream effectors in WT, [tert], and [tert x p53−/−] fish at 15 dpf. (a) wnk1a, (b) osr1a, (c) osr1b, (d) stk39, (e) ppp2r1ba, and (f) ppp2r1bb in 15 dpf [tert] and [tert x p53−/−] transgenic fish compared to WT. We performed three replicates, and for each qPCR, there were triplicates for each sample. Ten larvae were included in each experiment. One-way ANOVA analysis followed by multiple analysis was used to identify the statistical significance between groups. *: p ≦ 0.05; **: p ≦ 0.01; ****: p ≦ 0.0001; ns: no-significance.