Figure 3
The expressions of wnk1a and osr1b are upregulated in endothelial cells during tumor-induced angiogenesis. The expression of wnk1a and its downstream effectors during tumor-induced angiogenesis. (a) wnk1a, (b) osr1a, (c) osr1b, (d) stk39, (e) ppp2r1ba and (f) ppp2r1bb in zebrafish embryos from 0 dpi to 3 dpi during tumor-induced angiogenesis. We performed three to four replicates, and for each qPCR, there were triplicates for each sample. Thirty embryos were included in each experiment. One-way ANOVA analysis followed by multiple analysis was used to identify the statistical significance between groups. *: p ≦ 0.05; **: p ≦ 0.01; ***: p ≦ 0.001; ns: no-significance.