Figure 6

Col2a1a MO knockdown differentially affected ocular neural crest cell Sox10 and Foxd3 expression during early eye development. FL microscopy analysis of the effects of Col2a1a knockdown on GFP reporter expression in transverse cephalic sections from 48 hpf Tg (foxd3::EGFP) and Tg(sox10::EGFP) transgenic zebrafish embryos injected at the single-cell stage with a lissamine-tagged antisense prMO targeting col2a1a. MO knockdown of Col2a1a adversely affected Sox10-positive GFP expression in ocular anterior segment neural crest cells (A’–C’). The Foxd3-positive GFP expression in ocular neural crest cells was not affected by Col2a1a knockdown (A–C,C”,D). The coinjection of human (Hs) COL2A1 protein rescued Sox10-positive GFP expression (C’’’), while the injection of HsCOL2A1 alone (D’) adversely affected ocular Sox10-positive GFP expression in neural crest cells.