ZFIN Image: Liu et al., 2022, Figure 2

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Figure Caption

Figure 2

Knockout of the tmem39b gene sensitized zebrafish larvae to lethal cold stress. (A) Genotypes of the mutant lines. The mutations result in truncated proteins containing only 2 transmembrane domains. The genotypes are confirmed by DNA sequencing. The red box indicates the target sequence and the red arrows demonstrate the mutation sites. (B) Photos of the WT and zko3151b mutant larvae following exposure to cold-warming stress. The larvae were exposed to 10 °C for 24 h and photos were taken following the return to 28 °C for 12h. The red arrows point to dead fish. (C) Survival rates of WT and zko3151b mutant larvae following exposure to 10 °C for the indicated time points followed by recovery at 28 °C for 24 h. *, p < 0.05 (n = 4).

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Phenotype details

Acknowledgments

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