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FIGURE 3
KV morphogenesis and ciliogenesis in embryos treated with mild cold stress. (A,B) Morphology of KV. a1, normal KV at 28.5°C (100%, n = 133); a2, smaller KV at 22°C (63.6%, n = 137, p < 0.001); a3, tiny/absent KV at 22°C (36.4%, n = 137, p < 0.002). (C) Number and length of cilia. C1, cilia at 28.5°C (92.3%, n = 13); c2, cilia at 22°C (61.5%, n = 13, p < 0.001); c3, cilia at 22°C (38.5%, n = 13, p < 0.003). (D) Statistical chart for cilia number in KV. n = 13, p < 0.001 (E) Statistical chart for cilia length in KV. n = 12. Ns, not significant; “***“p < 0.001.
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