Fig. 5

Fig. 5

Figure 5. Molecular characteristics of neurons rapidly recruited to the injury

(A) Schematic cross section of the spinal cord showing dorsoventral domains.

(B) Maximum projection side views of olig2:EGFP (green) and mnx1:mTagBFP (red) fish at 1 DPL showing recruitment of ventral cell lineages to the injury. Arrowheads downward show olig2:EGFP⁺ cells and arrowheads upward show mnx1:mTagBFP⁺ neurons.

(C) Hb9 (Mnx1) and Islet1/2 immunohistochemistry showing recruitment of ventral cells to the injury at 1 DPL.

(D) Sagittal view showing slow recruitment of interneurons expressing vsx2:RFP to the lesion site between 1–3 DPL.

(E) Sagittal view showing marginal recruitment of dorsal inhibitory sensory neurons using the ptf1a:DsRed reporter between 1–3 DPL.

(F and G) Sagittal view showing abundant Nkx6.1⁺ but no Pax2⁺ immunolabelled cells at the lesion site 2 DPL. Spinal cord boundaries are outlined in yellow.

(H) Maximum projection of pax7a:EGFP labeling dorsal cell lineages at 2 DPL. The lesion site is outlined in white and is devoid of GFP⁺ cells. Scale bar, 50 μm.