ZFIN Image: Xiao et al., 2022, Fig. 5

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Figure Caption

Fig. 5

Comparison of neuron polarity among different neural sphere groups. (A–C) Nerve fiber phenotypes of neural spheres in different groups. (D–F) Partial enlarged drawing of (A–C) separately. (G–I) IF (Tuj1) showed the nerve fibers and neurites of neural spheres. Red arrow, nerve fiber; yellow arrowhead, neurite. (J) Comparison of average number of nerve fiber connections. (K) Comparison of average number of neurite outgrowth per neural sphere. **, p < 0.01; ***, p < 0.001, one-way ANOVA with Bonferroni post hoc, n = 3. Knockdown, infected with Doc2a-lentivirus; negative control, Lentivirus Luciferase Reporter negative control; blank control, cultured with an equal medium. Abbreviations: IF, immunofluorescence; DAPI, 4’,6-diamidino-2-phenylindole.

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