Fig. 1

Figure 1. The toxicity effects of As₂O₃ on zebrafish (Danio rerio) embryos. (A) Exposure to As₂O₃ increased embryo mortality in a dose-dependent manner. (B) LC₅₀ for As₂O₃. (C) Exposure to 30 µM and 40 µM As₂O₃ significantly decreased the incidence of tail coiling in 24 hpf embryos. (D) Exposure to 40 µM As₂O₃ significantly decreased the heartbeat of embryos examined at 48 hpf. (E) Exposure to increased As₂O₃ concentrations significantly delayed hatching between 48 hpf and 72 hpf. Data are presented as mean ± SEM of triplicate wells (n = 90 embryos per exposure group), with significant differences relative to the control group. ∗ (p ≤ 0.05); ^a no tail coiling, and heartbeats were recorded for 50 µM As₂O₃ exposed embryos, as all embryos were dead at 24 hpf.