ZFIN Image: Lou et al., 2022, Fig. 1

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Figure Caption

Fig. 1

Mafba and Mafbb regulate microglia development cell-autonomously during zebrafish early development. (A) Representative images of microglia in the optic tectum (OT) in 3 dpf siblings, mafba mutants, mafbb mutants, and DMut embryos in Tg(mpeg1:DsRedx) transgenic background. Microglia are labeled in DsRed. Dashed lines indicate the optic tectum region. (B) Quantification of microglia number in the OT in 3 dpf siblings (n = 7), mafba mutants (n = 7), mafbb mutants (n = 3), and DMut (n = 7) (mean ± SD; Student’s t test; nonsignificant [ns] P > 0.05, ****P < 0.0001). (C) Representative images of microglia in the OT in 3 dpf siblings, DMut embryos, DMut;Tg(mfap4:mafba-P2a-DsRedx) transgenic embryos, and DMut;Tg(mfap4:mafbb-P2a-DsRedx) transgenic embryos. Microglia are labeled in green color in Tg(mpeg1:eGFP) transgenic background. The overexpression of mafba and mafbb is indicated by red color in Tg(mfap4:mafba-P2a-DsRedx) and Tg(mfap4:mafbb-P2a-DsRedx) transgenic lines, respectively. Dashed lines indicate OT region. (D and E) Quantification of microglia in the OT in 3 dpf siblings (n = 6 or 7), mafba mutants (n = 4), mafbb mutants (n = 3), DMut (n = 10 or 8), DMut;Tg(mfap4:mafba-P2a-DsRedx) transgenic embryos (n = 12), and DMut;Tg(mfap4:mafbb-P2a-DsRedx) transgenic embryos (n = 16) (mean ± SD; Student’s t test; nonsignificant [ns] P > 0.05, **P < 0.01, ****P < 0.0001).

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA