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Fig. 8
Tnks inhibition amends dysregulation of cardiac Wnt signaling and miRNAs induced by ISO in adult zebrafish. (A) qRT-PCR analysis of axin2 and wnt4 expression in zebrafish hearts treated with ISO alone or together with XAV939 compared to the vehicle. (B) qRT-PCR analysis of axin2 and wnt4 expression in zebrafish hearts treated with XAV939 compared to the vehicle. (C) qRT-PCR analysis of col1a1a and col5a1 expression in zebrafish hearts treated with ISO alone or together with XAV939 compared to the vehicle. (D) qRT-PCR analysis of the expression of selected miRNAs in zebrafish hearts treated with ISO alone or together with XAV9393 compared to the vehicle. (E) qRT-PCR analysis of the expression of the selected miRNAs in zebrafish hearts treated with XAV939 compared to the vehicle. The graphs represent the quantification of two individual analyses of RNA extracts from pooling samples of three hearts. Each analysis includes three replicates. (A,C,D): vehicle, n = 18 hearts; ISO, n = 18 hearts; ISO+XAV939, n = 10 hearts. (B,E): vehicle, n = 10 hearts; XAV939, n = 10 hearts. Data are presented as mean ± SD. One-way ANOVA with Tukey adjustment for multiple comparisons (A,C,D). Two-sample t-test (B,E): * p < 0.05, ** p < 0.01.