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Fig. 1 Fig. 1. Genotyping workflow using the Agilent 5300 Fragment Analyzer. Clip a small piece of an adult zebrafish fin (or use a whole embryo) and extract the gDNA. Perform standard PCR using the gDNA and primers that flank the target region of interest. Run the PCR samples on the Agilent 5300 Fragment Analyzer, then analyze the DNA band sizes. The entire protocol—from gDNA extraction to data analysis—can be completed in 4 h (Figure was made using BioRender.com.)
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Reprinted from Developmental Biology, 484, Colijn, S., Yin, Y., Stratman, A.N., High-throughput methodology to identify CRISPR-generated Danio rerio mutants using fragment analysis with unmodified PCR products, 22-29, Copyright (2022) with permission from Elsevier. Full text @ Dev. Biol.