ZFIN Image: Deng et al., 2022, FIG 3

Image

Figure Caption

FIG 3

Adherence capacity, biofilm formation and exopolysaccharide (EPS) production of the LGG wild type versus △luxS in the presence or absence of exogenous, synthesized AI-2 (1 μM). (A–B) Adherence of LGG strains to IPEC-J2 cells. Five fields of vision were randomly selected for each slide under oil microscope to calculate the number of bacteria adhered to the surface of the visible cells, n = 6. (C) Biofilm determined by crystal violet staining, n = 6. (D) EPS produced in the biofilm matrix, n = 6. (E-G) Confocal laser scanning microscopy (CLSM) staining of living cells, dead cells, and EPS by SYTO9 (green), PI (red) and calcofluor white (blue), respectively, n = 3. AI-2, autoinducer-2; WT, wild type; ΔluxS, luxS mutant; WT/AI-2, wild-type strain supplemented with AI-2; ΔluxS/AI-2, ΔluxS strain supplemented with AI-2. P < 0.05 (*), P < 0.01 (**) and P < 0.001 (***).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Microbiol Spectr