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Fig. 7
Effect of Cinnamon Bark and Polygala Root on BDNF/CREB in NPY-KO zebrafish. mRNA levels of the (A,B) HPA axis, (C) SAM axis, (D,E) GABA -related gene, and (F) bdnf were estimated by real-time PCR using whole brains of the control—and Cinnamon Bark -and Polygala Root-fed NPY-KO zebrafish. (A) gr. (B) pomc. (C) th1. (D) gad1d. (E) gad2. (F) bdnf. The gene expression level of actb was used as an internal reference. Each gene expression level was relative to that in the control-fed NPY-KO zebrafish. n = 5. The protein levels of (G,H) p-CREB and (G,I) CREB were analyzed by immunoblotting with whole-brain lysate of the control—and herbal medicine-fed NPY-KO zebrafish. A loading control was used as the (H) total CREB and (I) β-actin. n = 8. Results are shown as mean ± standard error of the mean. There were significant differences between the different signs. n.s., not significant.