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Fig. 6
Evaluation of improvement of sociability in herbal medicine originating from NYT. (A–E) NPY-KO zebrafish were fed a control and herbal medicine diet twice a day for 4 days. Sociability was analyzed using the 3-chambers test. (A) Total distance traveled by control—and herbal medicine-fed NPY-KO zebrafish (equivalent to 3% NYT). (B) Tracking of control (left), Cinnamon Bark (middle), and Polygala Root-fed (right) swimming behavior. (C)Time spent in the control and herbal medicine-fed NPY-KO zebrafish in the fish chamber area. Control, n = 41; herbal medicines, n = 8. (D) Tracking of control (left), Cinnamon Bark (middle), and Polygala Root fed (middle), NYT (right) swimming behavior (equivalent to 0.3% NYT). (E) Time spent in the fish chamber area in control, Cinnamon Bark, Polygala Root, and NYT-fed NPY-KO zebrafish. Control n = 8; herbal medicines n = 7. Results are shown as mean ± standard error of the mean. There were significant differences between the different signs. n.s., not significant.