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Fig. 3
Effect of NYT on sociability in NPY-KO zebrafish. Wild and NPY-KO zebrafish were fed either control or NYT twice a day for 4 days. Sociability was analyzed using the 3-chambers test. (A) Test apparatus. (B) Experimental scheme for behavioral evaluation. (C) Tracking of wild (left) and NPY-KO zebrafish (right) swimming behavior. (D) Time spent in the fish chamber area in wild and NPY-KO zebrafish. (E) Total distance traveled in the WT and NPY-KO zebrafish. (F) Time spent in the empty chamber area of wild and NPY-KO zebrafish. Wild, n = 9; NPY-KO, n = 8. (G) Tracking of control (left) and NYT-fed (right) NPY-KO zebrafish swimming behavior. (H) Time spent in the fish chamber area of the control and NYT-fed NPY-KO zebrafish. (I) Total distance traveled by control and NYT-fed NPY-KO zebrafish. (J) Time spent in the empty chamber area in control and NYT-fed NPY-KO zebrafish. n = 8. Results are shown as mean ± standard error of the mean. n.s., not significant.