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Fig. 4
Figure 4. β cell-derived Cxcl8a is required for β cell loss in zMIR fish (A) Representative images of intra-islet macrophages in control and zMIR fish treated with DMSO (vehicle), TUDCA, and 4-PBA at 66 hpt. Red cells are islet β cells, and white cells are macrophages. Scale bars, 10 μm. (B) Intra-islet macrophage numbers in control and zMIR fish treated with DMSO (vehicle), TUDCA, and 4-PBA at 66 hpt. Data are mean ± SEM. n = 10/group, two-way ANOVA followed by Tukey’s multiple comparisons test; ∗∗∗p < 0.001. (C) Islet qRT-PCR analysis of cxcl8a at 66 hpt in control and zMIR fish treated with DMSO (vehicle), TUDCA, and 4-PBA at 66 hpt. Data are mean ± SEM. n = 3/group, two-way ANOVA followed by Tukey’s multiple comparisons test; ∗∗p < 0.01. (D) β Cell numbers in zMIR fish with or without cxcl8a function. Data are mean ± SEM. n = 15/group, two-way ANOVA followed by Tukey’s multiple comparisons test; ∗p < 0.05. (E) β Cell numbers in cxcl8a−/−, zMIR fish with cxcl8a rescue in β cells or macrophages. The rescue was achieved by Tg(ins:cxcl8a-P2A-nEGFP) and Tg(mpeg1:cxcl8a-P2A-tagRFPcaax), respectively. Data are mean ± SEM. n > 10/group, two-way ANOVA followed by Tukey’s multiple comparisons test; ∗p < 0.05.