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Fig. 1
EAAT2a is predominantly expressed in astroglial cells. (a, b) mRNA of eaat2a was expressed along the periventricular zones (TVe, TeVe, RVe), resembling astroglial localization patterns in larval zebrafish as visible in lateral (a, 3 dpf) and dorsal (b, 5 dpf) view. (c, d) Cross sections of (b) indicated by dashed lines. (e, f) eaat2a mRNA expression along periventricular zones (TVe, PGZ) was maintained in adult zebrafish anterior forebrain (e) and midbrain (f). (g–i) Protein expression of EAAT2a (magenta), glutamine synthetase (GS, cyan) and synaptic vesicle 2/acetylated tubulin (SV2/acT, yellow) on cross sections of larval anterior forebrain indicated in (b). (j–l) Overlay of EAAT2a, GS and SV2/acT showed a greater co‐localization of EAAT2a with astroglial (k) than neuronal (l) cells. CeP, cerebellar plate; Hi, intermediate hypothalamus; Hd, dorsal zone of periventricular hypothalamus; LVe, lateral ventricular recess of hypothalamus; OB, olfactory bulb nuclei; PGZ, periventricular gray zone of the optic tectum; TVe, periventricular zone of the telencephalon; TeO, optic tectum; TeVe, periventricular zone of the tectum; RVe, periventricular zone of the rhombencephalon. Scale bars are 200 μm in (a), (b), (e) and (f); 50 μm in (c) and (d); 10 μm in (g)