|
Fig. 5 Figure 5. OTUD3 promotes viral DNA-triggered signaling (A) qRT-PCR analysis of IFNB1, ISG56, CXCL10, and IL6 in the control or OTUD3-overexpressed THP-1 cells un-infected or infected with HSV for the indicated times. (B) qRT-PCR analysis of IFNB1, ISG56, CXCL10, and IL6 in the control and OTUD3 knockdown THP-1 cells un-infected or infected with HSV-1 for the indicated times. (C) qRT-PCR analysis of IFNB1, ISG56, CXCL10, and IL6 in the control and OTUD3 knockdown THP-1 cells un-transfected or transfected with ISD45 for 6 h. (D) ELISA of IFN-β in supernatant of the control or OTUD3 knockdown THP-1 cells infected with HSV for 0–24 h (top), mock transfected or transfected with ISD45 for 12 h (bottom). (E) IB of proteins in the control or OTUD3 knockdown THP-1 cells infected with HSV-1 for the indicated times by the indicated antibodies. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001, using two-way ANOVA with Holm-Sidak’s multiple comparisons test (A–D). Data based on one representative experiment performed in three biological replicates from at least three independent experiments (mean ± SD) or representative data (E).