ZFIN Image: Bruton et al., 2022, Fig. 4

Image

Figure Caption

Fig. 4

Figure 4. Macrophage ablation abolishes injury-dependent cardiomyocyte proliferationEdU pulse strategy for labeling proliferating cardiomyocytes over 0–24 hpi (left) and 24–48 hpi (right).

(B) Images of EdU-stained hearts from Tg(myl7:h2b-GFP) larvae at 48 hpi. Non-myocardial EdU signal is excluded post-acquisitionally. A, atrium; v, ventricle; white boxes, zoom panels; white arrowheads, EdU+ cardiomyocyte nuclei; dashed line, outline of dividing cardiomyocyte daughter nuclei.

(C) Percentage of ventricular EdU+ cardiomyocytes in uninjured and injured Tg(myl7:h2b-GFP) hearts pulsed over 0–24 or 24–48 hpi. ^∗∗∗ p ≤0.001 unpaired t test, n = 10–25.

(D) EdU pulse strategy for labeling proliferating cardiomyocytes over 24–48 hpi in Tg(myl7:h2b-GFP;csfr1a:NfsB-mCherry) larvae per standard macrophage ablation groups.

(E) Images of EdU-stained hearts from Tg(myl7:h2b-GFP;csfr1a:NfsB-mCherry) acquired by LSFM at 48 hpi. White boxes, zoom panels; white arrowheads, EdU+ cardiomyocyte nuclei; BA, bulbous arteriosus.

(F) Percentage of ventricular EdU+ cardiomyocytes in uninjured and injured Tg(myl7:h2b-GFP;csfr1a:NfsB-mCherry) hearts pulsed over 24–48 hpi. ^∗p ≤ 0.05 Kruskal-Wallis test and Dunn’s multiple comparison post-hoc test, n = 12–19.

(G) Images of uninjured and injured EdU-stained hearts from irf8^−/− Tg(myl7:h2b-GFP) acquired by LSFM at 48 hpi. Non-myocardial EdU signal is excluded post-acquisition to allow interpretable maximal intensity projections.

(H) Percentage of ventricular EdU+ cardiomyocytes in uninjured and injured irf8^+/+ and irf8^−/− Tg(myl7:h2b-GFP) hearts pulsed 24–48 hpi, ^∗∗ p ≤ 0.01 unpaired t test, n = 10–16. All images are maximum intensity projections of 3D LSFM stacks, unless otherwise stated. Scale bars, 50 μm. Data are represented as mean ± SEM.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 57(12), Bruton, F.A., Kaveh, A., Ross-Stewart, K.M., Matrone, G., Oremek, M.E.M., Solomonidis, E.G., Tucker, C.S., Mullins, J.J., Lucas, C.D., Brittan, M., Taylor, J.M., Rossi, A.G., Denvir, M.A., Macrophages trigger cardiomyocyte proliferation by increasing epicardial vegfaa expression during larval zebrafish heart regeneration, 1512-1528.e5, Copyright (2022) with permission from Elsevier. Full text @ Dev. Cell