ZFIN Image: Lu et al., 2022, FIGURE 5

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Figure Caption

FIGURE 5

Knockdown of cingulin b reduces the number of HCs and cell proliferation in the neuromasts of zebrafish at 72 hpf. (A–H) The immunochemical staining of PLL neuromasts in the Control-MO group (n = 19) and cingulin b-MO group (n = 15). DAPI (green) labels nuclei (A,E) and BrdU (red) labels proliferative cells in the neuromast (B,F). In transgenic Tg (brn3c: mGFP)^s^356t lines, the membrane of HCs in PLL neuromasts are labeled with green fluorescence (GFP) (C,G). (I) The average number of hair cells per neuromast is significantly reduced after inhibition of cingulin b. (J) BrdU index in the neuromasts is also severely downregulated in the cingulin b-MO injected embryos compared to the controls. Scale bars mark 10 μm (A–H). Data are shown in mean ± SEM, and ****p < 0.0001. (K) The differentiation of HCs indicated by atoh1 ISH staining is inhibited after injection with cingulin b-MO. Scale bars in panel (K) mark 30 μm.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.