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Fig. 1
Time-course analysis of Gt(endocard:egfp) fluorescence demonstrates enriched endocardial expression at somitogenesis stages in the zebrafish embryo. Immunofluorescence staining of Gt(endocard:egfp); Tg(kdrl:Hsa.HRAS-mCherry) and Tg(fli1a:egfp) embryos from 10 s through to 48 hpf. Anterior to the top. Anterior views at 48 hpf, all other images show dorsal views. White arrowheads label kdrl+/endocard:egfp+ endocardial cells. White arrows label kdrl+/endocard:egfp− vascular endothelial cells. Black arrowheads label presumptive myocardial cells. Scale bars: 50 μm.