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Figure 1
Figure 1. Spatiotemporal expression pattern of slc38a9 in zebrafish. (A) RT-PCR analysis of zebrafish slc38a9 mRNA at the indicated embryonic stages. Numbers indicate different developmental stages as hours post-fertilization. NC, negative control. β-actin as the internal control. (B–L) Wholemount in situ hybridization (WISH) reveals the slc38a9 mRNA expression pattern in the zebrafish embryos at the indicated embryonic stages. slc38a9 expression is ubiquitous from the one-cell stage to 12 hpf (B–E), restricted to the retina at 18 (F), 24 (G), and 30 hpf (H). The slc38a9 expression encompasses the heart and midbrain region at 30 (H) and 48 hpf (I). From 72 hpf to 5 dpf, the slc38a9 expression is restricted to the liver and gut (J,L). (M,N) Views of zebrafish embryos hybridized with sense zebrafish slc38a9 probe. Abbreviations: e, eye, mb, midbrain; g, gut; hb, hindbrain; l, liver; h, heart.
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Full text @ Int. J. Mol. Sci.