Figure 4

Figure 4. PCNA staining of proliferation: (i) (A) Proliferation is minimal in the control uninjured retina (0 dpi). (B) 1 dpi: proliferation begins to be detected (arrows). (C,D) 2 dpi and 3 dpi: proliferation more evident at the lesion site. (E) 5 dpi: proliferation pronounced, with positive nuclei located predominantly in the ONL at the lesion margin, absent in the lesion site (circled). (F) 7 dpi: proliferation now detected throughout the lesion. (G,H) 10 dpi and 20 dpi: proliferation started to decrease as new cells have started to repopulate the lesion site. (I,J) 30 dpi and 60 dpi: proliferation now only marginally detected (arrows). All sections were counterstained with DAPI. PCNA proliferation marker—red; Nuclei, DAPI—blue. Retinal layers labelled in control panel for reference; RPE—retinal pigment epithelium, ONL—outer nuclear layer, OPL—outer plexiform layer, INL—inner nuclear layer, IPL—inner plexiform layer, GCL—ganglion cell layer. Scale bars represent 200 µm. (ii) Relative percentage of proliferative cells at each time-point (n = 2), * p value < 0.05.