ZFIN Image: Kulkarni et al., 2022, Figure 6

Image

Figure Caption

Figure 6

Pioglitazone treatment reduces lipid droplet accumulation in the liver. (A) Experimental scheme of PIO treatment. Larvae were immersed in PIO+ 0.1% DMSO for 1 h prior to the first HFD treatment and remained in PIO throughout. (B–D) Representative optical slices of BODIPY-stained 2CLIP zebrafish liver depicting (B) untreated controls, (C) HFD-fed larvae, and (D) HFD-fed + PIO hi (5 µM) treated larvae; hepatocytes are labeled in red via expression of the transgene fabp10:dsRed and examples of green-labeled lipid droplets are indicated by arrows. (E) Quantification of lipid droplets in hepatocytes area as executed with FIJI software. Statistical analysis was by one-way ANOVA; * p < 0.05, ** p < 0.01. Scale bar = 50 µm.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biomedicines