Figure 4—figure supplement 1.

Figure 4—figure supplement 1.

(A,B) ryk morphants present Convergent Extension (CE) defects that can be rescued by WT ryk (A) but not ryk^nce4g mutant (B) RNA. (C) CE is similar in Zygotic (Z) ryk^nce4g mutants and their WT siblings. (D) In situ hybridization reveals that ryk transcript levels are reduced in Z ryk^nce4g mutants (n = 24) compared to WT siblings (n = 24). 12 somite stage embryos, anterior to the left, dorsal up. To warrant identical acquisition conditions, two embryos were photographed on a single picture. (E–F) foxa3 in situ hybridization shows that Maternal Zygotic (MZ) ryk mutants present a reduced axial elongation (E) and an increased width of the notochord (F, for quantification see Figure 4I). (G) qPCR analysis of bud stage embryos reveals that MZ ryk^nce4g mutants present reduced ryk transcript levels. (H) In contrast to Z ryk^nce4g mutants, Maternal Zygotic (MZ) ryk^nce4g mutants present CE defects. To exclude any defects due to genetic background variation, the parental fish used to obtain the embryos for this experiment were ryk[+/+] and ryk[nce4g/nce4g] siblings obtained from the same incross. ryk WT RNA injection allows to rescue MZ ryk^nce4g mutant CE defects. (I) ryk morpholino injection has no effect in MZ ryk^nce4g mutants. Lateral (A,B,C,E,H,I) or dorsal (F) views of bud stage embryos, anterior up. Scalebars 200 µm. Boxes in (E,I) boxes represent mean values ± SD. Error bars in G represent SE from three biological replicates. Quantitative analysis of the data sets displayed in (A,B,C,H) is provided in Figure 4D, E, G and H respectively. See Figure 4—source data 1 for complete statistical information.