Figure 1—figure supplement 3.

Figure 1—figure supplement 3.

(A) Mib1 morphants display reduced convergent extension (quantified through the measure of the axis extension level α) that is not rescued by constitutively activated Notch (NICD). (B) cDNA sequence from wild-type embryos injected with mib1 exon/intron1 splice morpholino. Morpholino injection causes a retention of intron 1. As a consequence, the Mib1 morphant proteins comprises only the first 76 amino acids of WT Mib1 followed by 14 intronically encoded residues and a premature Stop codon. (C) Mib1 morphant axis extension can be restored through the overexpression of Notch-signaling-deficient mib1^ta52b. (A,C) depict lateral views of bud stage embryos, anterior up, dorsal to the right. A quantitative analysis of the corresponding data sets is provided in Figure 1E and F. Scalebars: 200 µm.