(A–C) Microinjection of RNA encoding the Mib1ta52b mutant protein induces Ryk internalisation (B, n = 16/23 embryos) or degradation (C, n = 7/23 embryos) as compared to WT controls (A, n = 21). Dorsal views of 90% epiboly stage embryos, anterior up. The Histone2B-mRFP signal (A’-C’) was used to ascertain that control and Mib1ta52b-expressing embryos had received comparable amounts of injected material. Scalebar: 20 µm.
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