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Fig 3
Rods were labeled with EGFP by crossing the WT or nrl-KO zebrafish with the Tg(rho:EGFP) transgenic line. Fluorescence was observed every 2 days from 3 dpf. Representative images of WT and nrl-KO retinas are shown in (A) for 3–9 dpf and (B) for 11–17 dpf. The dotted circles indicate the boundaries of the retinas. D, dorsal; V, ventral; N, nasal; T, temporal. Scale bars: 100 μm. (C) Immunostaining of retinal sections for Gnat1 (the rod transducin alpha-subunit) showed exact co-localization with EGFP in rods at 7 dpf. Rods were barely observed in the nrl-KO retinas. Scale bar: 50 μm.
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