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Figure 4
Figure 4. Stronger and slower neuronal activity in the pretectum area in mct8−25bp−/− larvae. (A) Dorsal view of tg(HuC:H2B-GCaMP6s) 6 dpf larva (head pointing to the top). The dashed yellow square marks the enlarged view of the pretectum shown in (B). Scale bar = 50 µm. (C) Average relative fluorescence variation (∆F/F) activity of [mct8−25bp−/− × tg(HuC:H2B-GCaMP6s)] (grey) and their tg(HuC:H2B-GCaMP6s)-siblings (black). (D–G) Changes in single-cell Ca2+ events are presented by the average number of events (D), frequency (E), amplitude (F) and duration (G). The ‘+’ sign shows the mean. tg(HuC:H2B-GCaMP6s)-siblings: n = 9, [mct8−25bp−/− × tg(HuC:H2B-GCaMP6s)]: n = 6). Statistical significance was determined by Mann–Whitney U test. * (p < 0.05).
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Full text @ Int. J. Mol. Sci.