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Figure Caption
FIGURE 1
CRISPR-Cas9 generated a 27 bp insertion that disrupts the ankk1 sequence (A) Comparison of wild type (top) and mutant (bottom) ankk1 sequences. crRNA is highlighted in blue. PAM sequence is highlighted in yellow. The restriction site (that is disrupted in the F0 screening) appears in red, underlined. (B) Comparison of wild type (top) and mutant (bottom) amino acid sequences. Mutant sequence generates an early stop codon.
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Full text @ Front. Neurosci.