ZFIN Image: Paulissen et al., 2022, Figure 6

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Figure Caption

Figure 6

Retinoic acid loss contributes to changes in the definitive vasculature, resulting in large veins and small arteries.

(A, B) A 24 hpf tg(kdrl:eGFP) embryo labeled with DAPI. (A) Wild-type embryos show lumenized blood vessels and normal-sized dorsal aorta. (B) Labeled aldh1a2-/-, tg(kdrl:eGFP) sibling shows small lumenized dorsal aorta with large posterior cardinal vein. (C) A 5 dpf tg(kdrl:eGFP) embryo. White arrows indicate the dorsal aorta, and yellow arrowheads indicate the cardinal vein. (D) A 5 dpf tg(kdrl:eGFP), aldh1a2-/- embryo. White arrows indicate reduced size of dorsal aorta. (E) Quantification of DMSO (n = 12) or 20 μM DEAB (n = 12)-treated tg(kdrl:nls-eGFP) embryos. Nuclei were counted in each structure over a 225 μM long transverse section of the zebrafish trunk. Two-tailed p-values for the unpaired t-test of wild-type vs. 20 μM DEAB-treated embryos are <0.00001 and 0.0002 for arteries and veins, respectively. Scale bars, 50 µm.

Acknowledgments

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