ZFIN Image: Jin et al., 2021, Fig. 5

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Fig. 5 Immunoprecipitation analysis of YARS2 with subunits of OXPHOS complexes. A, co-immunoprecipitation of YARS2-FLAG and subunits of OXPHOS. HeLa cells transiently expressing with or without YARS2-FLAG were solubilized with a lysis buffer and lysate proteins were immunoprecipitated with immunocapture buffer (left) (input) and FLAG-antibody (right) (IP), respectively. Immunoprecipitates were analyzed by SDS-PAGE and western blotting using anti-FLAG, anti-subunits of OXPHOS (NDUFS1, NDUFS3, NDUFA9 and NDUFB8 for complex I, SDHB for complex II, UQCRC1 and UQCRC2 for complex III, CO2, COX4, COX5A, COX10, COX16, and COX17 for complex IV, ATP8 and ATP5F1 for complex V) and GAPDH antibodies, respectively. B and C, co-immunoprecipitation of YARS2-HA and NDUFS1, CO2 using anti-HA, anti-NDUFS1, CO2, and GAPDH antibodies, respectively. D, co-immunoprecipitation of YARS2-HA and NDUFS9A-FLAG using anti-HA, anti-FLAG, and GAPDH antibodies, respectively. E, co-immunoprecipitation of HARS2-FLAG and NDUFS1, NDUFA9, CO2 in lysates of HeLa cells transfected with plasmids containing HARS2 using anti-FLAG, anti-NDUFS1, NDUFA9, and CO2 antibodies respectively.

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