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Fig. 1 Knockdown and knockout of cbs or cth impair brain vascular development of larval zebrafish. (A) In situ hybridisation of whole zebrafish larvae at 3 dpf showing the ubiquitous expression of cbs and cth in the brain (lateral view). (B and C) Effects of morpholino oligonucleotide-mediated cbs or cth knockdown on brain vascular development. (B) Representative midbrain vessel structures reconstructed from confocal images of cbs or cth morphants at 3–5 dpf of the same larva. (C) Summary of data. The experiments were repeated three times, and six embryos were examined for each group at each time. (D and E) Effects of cbs or cth knockout (F0) on brain vascular development. (D) Representative midbrain vessel structures reconstructed from confocal images of the same F0 mutant larva at 3–5 dpf. (E) Summary of data. Six embryos were examined for each group. (F and G) Effects of cbs or cth homomutants on brain vascular development. (F) Representative midbrain vessel structures reconstructed from confocal images of the same cbs or cth homomutant larva at 3 and 5 dpf. (G) Summary of data. Six embryos were examined for each group. Scale bar, 300 µm (A), 25 µm (B, D, F). Error bars, SEM. *P<0.05, **p<0.01, ***p<0.001 (unpaired two-tailed Student’s t-test).