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Fig 10
Photoconversion experiments show that delamination defects in FK506-treated embryos results in misassignment of cells from luminal to abluminal cell fate.
(A) Flow diagram summarizing the method used in these photoconversion experiments. (B–B’) Representative images showing that DMSO treatment does not cause misassignment of cell fate. (C–C”) Representative images showing that FK506 treatment results in misassignment of cells from luminal to abluminal cell fate. Scale bars: 20 μm (D) Proposed fate map of FK506-treated embryos. Cells are colored to indicate position and fate over time. Asterisks denote cells that have been misassigned from luminal to abluminal cell fate and their progeny. hpf, hours postfertilization; VIC, valve interstitial cell.
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